MersV1, Main, Exploration, bibRecord, 004324

Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs

Identifieur interne : 004324 ( Main/Exploration ); précédent : 004323; suivant : 004325

Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs

Auteurs : Emanuelle Pascolo [France] ; Denis Hudrisier [France] ; Brian Sproat [Allemagne] ; Nguyen T. Thuong [France] ; Jean-Jacques Toulme [France]

Source :

BBA - Gene Structure and Expression [ 0167-4781 ] ; 1994.

RBID : ISTEX:3245568FFAB7443833309DB61736BFA3E1F3CD79

English descriptors

Teeft :
- Acad, Acta, Amazonensis, Antisense, Antisense effect, Antisense oligonucleotide, Antisense oligonucleotides, Base pairs, Biochimica, Biophysica, Biophysica acta, Denaturing polyacrylamide, Duplex, Electrophoretic analysis, Gene expression, Hairpin, Hairpin structure, Helper oligonucleotide, Leishmania, Leishmania amazonensis, Leishmania mrnas, Mrna, Natl, Nucleic, Nucleic acids, Oligomer, Oligomers, Oligonucleotide, Oligonucleotides, Pascolo, Polyacrylamide, Previous study, Proc, Protein synthesis, Psoralen, Psoralen derivatives, Rabbit reticulocyte lysate, Reticulocyte, Rnase, Simultaneous addition, Thuong, Toulm, Toulm6, Wheat germ.

Abstract

Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.

Url:

https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.pdf

DOI: 10.1016/0167-4781(94)90251-8

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001E13
to stream Istex, to step Curation: 001E13
to stream Istex, to step Checkpoint: 001961
to stream Main, to step Merge: 004387
to stream Main, to step Curation: 004324

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title>
<author><name sortKey="Pascolo, Emanuelle" sort="Pascolo, Emanuelle" uniqKey="Pascolo E" first="Emanuelle" last="Pascolo">Emanuelle Pascolo</name>
</author>
<author><name sortKey="Hudrisier, Denis" sort="Hudrisier, Denis" uniqKey="Hudrisier D" first="Denis" last="Hudrisier">Denis Hudrisier</name>
</author>
<author><name sortKey="Sproat, Brian" sort="Sproat, Brian" uniqKey="Sproat B" first="Brian" last="Sproat">Brian Sproat</name>
</author>
<author><name sortKey="Thuong, Nguyen T" sort="Thuong, Nguyen T" uniqKey="Thuong N" first="Nguyen T." last="Thuong">Nguyen T. Thuong</name>
</author>
<author><name sortKey="Toulme, Jean Jacques" sort="Toulme, Jean Jacques" uniqKey="Toulme J" first="Jean-Jacques" last="Toulme">Jean-Jacques Toulme</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:3245568FFAB7443833309DB61736BFA3E1F3CD79</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1016/0167-4781(94)90251-8</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-RHHJRVB1-G/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001E13</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001E13</idno>
<idno type="wicri:Area/Istex/Curation">001E13</idno>
<idno type="wicri:Area/Istex/Checkpoint">001961</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001961</idno>
<idno type="wicri:doubleKey">0167-4781:1994:Pascolo E:relative:contribution:of</idno>
<idno type="wicri:Area/Main/Merge">004387</idno>
<idno type="wicri:Area/Main/Curation">004324</idno>
<idno type="wicri:Area/Main/Exploration">004324</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs</title>
<author><name sortKey="Pascolo, Emanuelle" sort="Pascolo, Emanuelle" uniqKey="Pascolo E" first="Emanuelle" last="Pascolo">Emanuelle Pascolo</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Bordeaux cédex</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hudrisier, Denis" sort="Hudrisier, Denis" uniqKey="Hudrisier D" first="Denis" last="Hudrisier">Denis Hudrisier</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Sanofi Elf-Biorecherches, Labège Innopole, BP 137, 31676 Labège cédex</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Midi-Pyrénées</region>
<settlement type="city">Labège cédex</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sproat, Brian" sort="Sproat, Brian" uniqKey="Sproat B" first="Brian" last="Sproat">Brian Sproat</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>EMBL, Meyerhofstrasse 1, D-69012 Heidelberg</wicri:regionArea>
<wicri:noRegion>69012 Heidelberg</wicri:noRegion>
<wicri:noRegion>D-69012 Heidelberg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thuong, Nguyen T" sort="Thuong, Nguyen T" uniqKey="Thuong N" first="Nguyen T." last="Thuong">Nguyen T. Thuong</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Centre de Biophysique Moléculaire, CNRS, 45071 Orléans</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Orléans</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Toulme, Jean Jacques" sort="Toulme, Jean Jacques" uniqKey="Toulme J" first="Jean-Jacques" last="Toulme">Jean-Jacques Toulme</name>
<affiliation></affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biophysique Moléculaire, INSERM U386, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux cédex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Bordeaux cédex</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">BBA - Gene Structure and Expression</title>
<title level="j" type="abbrev">BBAEXP</title>
<idno type="ISSN">0167-4781</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1994">1994</date>
<biblScope unit="volume">1219</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="98">98</biblScope>
<biblScope unit="page" to="106">106</biblScope>
</imprint>
<idno type="ISSN">0167-4781</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-4781</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term>
<term>Acta</term>
<term>Amazonensis</term>
<term>Antisense</term>
<term>Antisense effect</term>
<term>Antisense oligonucleotide</term>
<term>Antisense oligonucleotides</term>
<term>Base pairs</term>
<term>Biochimica</term>
<term>Biophysica</term>
<term>Biophysica acta</term>
<term>Denaturing polyacrylamide</term>
<term>Duplex</term>
<term>Electrophoretic analysis</term>
<term>Gene expression</term>
<term>Hairpin</term>
<term>Hairpin structure</term>
<term>Helper oligonucleotide</term>
<term>Leishmania</term>
<term>Leishmania amazonensis</term>
<term>Leishmania mrnas</term>
<term>Mrna</term>
<term>Natl</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Oligomer</term>
<term>Oligomers</term>
<term>Oligonucleotide</term>
<term>Oligonucleotides</term>
<term>Pascolo</term>
<term>Polyacrylamide</term>
<term>Previous study</term>
<term>Proc</term>
<term>Protein synthesis</term>
<term>Psoralen</term>
<term>Psoralen derivatives</term>
<term>Rabbit reticulocyte lysate</term>
<term>Reticulocyte</term>
<term>Rnase</term>
<term>Simultaneous addition</term>
<term>Thuong</term>
<term>Toulm</term>
<term>Toulm6</term>
<term>Wheat germ</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: We investigated the properties of two antisense oligonucleotides, 11αPso and 14TMP, 11 and 14 nucleotides long, respectively, and conjugated to psoralen derivatives. These oligonucleotides were complementary to the mini-exon sequence of Leishmania amazonensis. Upon ultraviolet (UV) irradiation these oligomers were selectively cross-linked to DNA or RNA target sequences, either 14 or 35 nucleotides long. The yield of photo-addition was much lower on the longer targets than on the shorter ones, due to the presence of a hairpin structure. The co-addition of a helper oligonucleotide, whose binding site, on the 35-mer, was adjacent to that of the psoralen-derivatized antisense oligomer, improved the cross-linking efficiency. We then determined the effect of 14TMP on in vitro translation of Leishmania mRNA in cell-free extracts. Non-irradiated antisense oligonucleotide/mRNA complexes reduced the protein synthesis in wheat germ extract but not in rabbit reticulocyte lysate. Conversely, UV irradiation induced a 14TMP-dependent reduction of translation in reticulocyte lysate whereas the inhibition was not improved in the wheat germ extract. These results are discussed with respect to the involvement of RNase-H in the oligonucleotide-mediated effect on protein synthesis.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>France</li>
</country>
<region><li>Aquitaine</li>
<li>Centre-Val de Loire</li>
<li>Midi-Pyrénées</li>
<li>Nouvelle-Aquitaine</li>
<li>Occitanie (région administrative)</li>
<li>Région Centre</li>
</region>
<settlement><li>Bordeaux cédex</li>
<li>Labège cédex</li>
<li>Orléans</li>
</settlement>
</list>
<tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Pascolo, Emanuelle" sort="Pascolo, Emanuelle" uniqKey="Pascolo E" first="Emanuelle" last="Pascolo">Emanuelle Pascolo</name>
</region>
<name sortKey="Hudrisier, Denis" sort="Hudrisier, Denis" uniqKey="Hudrisier D" first="Denis" last="Hudrisier">Denis Hudrisier</name>
<name sortKey="Thuong, Nguyen T" sort="Thuong, Nguyen T" uniqKey="Thuong N" first="Nguyen T." last="Thuong">Nguyen T. Thuong</name>
<name sortKey="Toulme, Jean Jacques" sort="Toulme, Jean Jacques" uniqKey="Toulme J" first="Jean-Jacques" last="Toulme">Jean-Jacques Toulme</name>
</country>
<country name="Allemagne"><noRegion><name sortKey="Sproat, Brian" sort="Sproat, Brian" uniqKey="Sproat B" first="Brian" last="Sproat">Brian Sproat</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004324 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004324 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:3245568FFAB7443833309DB61736BFA3E1F3CD79
   |texte=   Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs

Relative contribution of photo-addition, helper oligonucleotide and RNase H to the antisense effect of psoralen-oligonucleotide conjugates, on in vitro translation of Leishmania mRNAs

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri